Ismaili Lhassane, Truong Tong Than, André Claire, Thomassin Mireille, Mozer Jean-Louis, Robert Jean-Francois, Xicluna Alain, Refouvelet Bernard, Millet Joelle, Nicod Laurence, Guillaume Yves-Claude
Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Equipe des Sciences Séparatives et Biopharmaceutiques (2SB) Place St-Jacques, 25030 Besançon Cedex, France.
J AOAC Int. 2003 Mar-Apr;86(2):222-8.
The mechanism of the binding of D,L dansyl amino acids to teicoplanin was investigated. Na+ was used as an indicator of the interactions between the solutes and teicoplanin. The number (n) of sodium ions, Na+, excluded from the solute-teicoplanin interface when analyte transfer occurred was determined. A thermodynamic study and enthalpy-entropy compensation were performed to further explore the interaction mechanism. From these results, it was shown that teicoplanin was balanced between 2 conformational states characterized by distinct enantioselective properties. This approach indicates that liquid chromatography (LC) is a useful tool to extract physicochemical and molecular information from retention data. Thus, LC can be used as a complementary technique with the conventional techniques of molecular interaction analysis.
