Forte Wilma Carvalho Neves, Almeida Rafael M, Bizuti Gilberto da Silva Cairo, Forte Daniel Neves, Bruno Simone, Russo Filho Francisco S, Lima Carlos Alberto Conceição
Departamento de Cl nica M dica, Hospital Central, Santa Casa de Miseric rdia de S o Paulo.
Rev Assoc Med Bras (1992). 2003 Jan-Mar;49(1):35-9. doi: 10.1590/s0104-42302003000100031. Epub 2003 Apr 28.
To evaluate the presence of immune complexes and the phagocytes by polymorphonuclear neutrophils in patients with systemic lupus erythematosus, with and without disease activity.
The peripheral blood of 55 subjects was analyzed. Ten of those subjects had disease activity, 15 had not disease activity, and 30 were healthy. We used radial immune diffusion to detect immune complexes. The phagocytic function was estimated by the ingestion of zymosan by polymorphonuclear neutrophils.
In this study we found the presence of immune complexes formatted of IgM, IgG, IgA, and complement component C3 and C4 in LES patients. The arithmetic average of zymosan particles ingested by the neutrophils incubated with homologous human serum and autologous human serum was significantly decreased (p<0.05) in the LES activity patients when we compare with the group without activity, and the control group.
We conclude that there are immune complexes in the LES patients with and without disease activity, and there is a reduction in the digestive step of the phagocytes by polymorphonuclear neutrophils in patients with disease activity. The conclusions of the present study are according with the pathogenesis of the disease and with the high mortality in these patients.
评估系统性红斑狼疮患者（无论有无疾病活动）体内免疫复合物的存在情况以及多形核中性粒细胞的吞噬细胞情况。
对55名受试者的外周血进行分析。其中10名受试者有疾病活动，15名无疾病活动，30名健康。我们采用放射免疫扩散法检测免疫复合物。通过多形核中性粒细胞对酵母聚糖的摄取来评估吞噬功能。
在本研究中，我们发现系统性红斑狼疮患者体内存在由IgM、IgG、IgA以及补体成分C3和C4组成的免疫复合物。与无疾病活动组和对照组相比，在疾病活动的系统性红斑狼疮患者中，与同源人血清和自体人血清孵育的中性粒细胞摄取的酵母聚糖颗粒算术平均值显著降低（p<0.05）。
我们得出结论，无论有无疾病活动，系统性红斑狼疮患者体内均存在免疫复合物，且疾病活动患者中多形核中性粒细胞对吞噬细胞的消化步骤减少。本研究的结论与该疾病的发病机制以及这些患者的高死亡率相符。
