Byrd Brian D, Heintzelman Douglas L, McNally-Heintzelman Karen M
Biomedical Engineering Program, Rose-Hulman Institute of Technology, Terre Haute, IN 47803, USA.
Biomed Sci Instrum. 2003;39:6-11.
The feasibility of using alternative chromophores in laser tissue soldering applications was explored. Two commonly used chromophores, indocyanine green (ICG), and methylene blue (MB) were investigated, as well as three different food colorings: red #40 (RFC), blue #1 (BFC), and green consisting of yellow #5 and blue #1 (GFC). Three experimental studies were conducted: (i) The absorption profiles of the five chromophores, when diluted in deionized water and when bound to protein, were recorded; (ii) the effect of accumulated thermal dosages on the absorption profile of the chromophores was evaluated; and (iii) the stability of the absorption profiles of the chromophore-doped solutions when exposed to ambient light for extended time periods was measured. The peak absorption wavelengths of ICG, MB, RFC, and BFC, were found to be 805 nm, 665 nm, 503 nm, and 630 nm respectively in protein solder. The GFC had two absorption peaks at 426 nm and 630 nm, corresponding to the two dye components comprising this color. The peak absorption wavelength of ICG and MB was dependent on the choice of solvent (deionized water or protein). In contrast, the peak absorption wavelengths of the three chromophores were not dependent on the choice of solvent. ICG and MB showed a significant decrease in absorbance units with increased time and temperature when heated to temperature up to 100 degrees C. A significant decrease in the absorption peak occurred in the ICG and MB samples when exposed to ambient light for a period of 7 days. Negligible change in absorption with accumulated thermal dose up to 100 degrees C or light dose (over a period of 84 days) was observed for any of the three food colorings investigated.
探讨了在激光组织焊接应用中使用替代发色团的可行性。研究了两种常用的发色团，吲哚菁绿（ICG）和亚甲蓝（MB），以及三种不同的食用色素：红色40号（RFC）、蓝色1号（BFC）和由黄色5号和蓝色1号组成的绿色（GFC）。进行了三项实验研究：（i）记录了五种发色团在去离子水中稀释以及与蛋白质结合时的吸收光谱；（ii）评估了累积热剂量对发色团吸收光谱的影响；（iii）测量了发色团掺杂溶液在长时间暴露于环境光下时吸收光谱的稳定性。在蛋白质焊料中，ICG、MB、RFC和BFC的峰值吸收波长分别为805nm、665nm、503nm和630nm。GFC在426nm和630nm处有两个吸收峰，分别对应于构成这种颜色的两种染料成分。ICG和MB的峰值吸收波长取决于溶剂的选择（去离子水或蛋白质）。相比之下，三种食用色素的峰值吸收波长不取决于溶剂的选择。当加热到100摄氏度时，ICG和MB的吸光度单位随时间和温度的增加而显著降低。当ICG和MB样品暴露于环境光7天时，吸收峰显著降低。在所研究的三种食用色素中，在累积热剂量高达100摄氏度或光剂量（在84天内）的情况下，观察到吸收变化可忽略不计。
