Butler Kenneth R, Benghuzzi Hamed A
University of Mississippi Medical Center, School of Health Related Professions, 2500 North State Street, Jackson, Mississippi 39216, USA.
Biomed Sci Instrum. 2003;39:535-40.
Recent studies in our laboratory have demonstrated the need for an experiment comparing the effect of steroid hormones on the cellular composition of the fibrous tissue capsules surrounding tricalcium phosphate (TCP) bioceramics. The effect of steroid hormones on inflammatory cells has been widely reported in the scientific literature. The purpose of this research is to contribute to the development of a long-term ceramic drug delivery system with human and veterinary applications. This study contributes information on the composition of fibrous tissue capsules surrounding hormone loaded TCP bioceramic implants at 90 days post-implantation. The data obtained in this study was gathered using protocols developed from ImagePro software. Sixteen animals in four experimental groups were implanted with one TCP bioceramic each. Group I animals were implanted with a sham TCP ceramic not containing a steroid hormone (control group). Group II animals received the testosterone loaded TCP ceramic. Group III animals were implanted with the dihydrotestosterone loaded ceramic. Group IV animals received the androstenedione ceramic. At 90 days post-implantation, the animals were euthanized. The implants and fibrous tissue capsules surrounding them were then extracted. The fibrous tissue capsules were evaluated microscopically using ImagePro software following routine II&E staining, modified Papanicolau, and Masson's trichrome. The research variables comparing the hormonal effects on fibrous tissue composition obtained by digital analysis were as follows: thickness of the fibrous tissue, quantification of macrophages, neutrophils, fibroblasts, vascularity, and area occupied by collagen. The results of this study support several conclusions. All three of the hormones in this study, particularly androstenedione, have significant affects on the thickness and cellular composition of the fibrous tissue capsules when compared to the control group. Digital analysis software enables more accurate and reproducible results to be obtained when specific procedures are followed.
我们实验室最近的研究表明，需要进行一项实验，比较类固醇激素对磷酸三钙（TCP）生物陶瓷周围纤维组织包膜细胞组成的影响。类固醇激素对炎症细胞的影响在科学文献中已有广泛报道。本研究的目的是为开发一种具有人类和兽医应用价值的长期陶瓷药物递送系统做出贡献。本研究提供了关于激素负载TCP生物陶瓷植入物植入后90天周围纤维组织包膜组成的信息。本研究中获得的数据是使用从ImagePro软件开发的方案收集的。四个实验组的16只动物分别植入一个TCP生物陶瓷。第一组动物植入不含类固醇激素的假TCP陶瓷（对照组）。第二组动物接受负载睾酮的TCP陶瓷。第三组动物植入负载二氢睾酮的陶瓷。第四组动物接受雄烯二酮陶瓷。植入后90天，对动物实施安乐死。然后取出植入物及其周围的纤维组织包膜。在常规II&E染色、改良巴氏染色和Masson三色染色后，使用ImagePro软件对纤维组织包膜进行显微镜评估。通过数字分析比较激素对纤维组织组成影响的研究变量如下：纤维组织厚度、巨噬细胞、中性粒细胞、成纤维细胞定量、血管分布以及胶原所占面积。本研究结果支持几个结论。与对照组相比，本研究中的所有三种激素，尤其是雄烯二酮，对纤维组织包膜的厚度和细胞组成都有显著影响。当遵循特定程序时，数字分析软件能够获得更准确和可重复的结果。
