Butler K, Puckett A, Benghuzzi H
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:267-72.
This investigation was specifically an attempt to correlate the thickness of this fibrous capsule and the various histological components that are seen in the fibrous capsule surrounding ALCAP, HA, and TCP ceramics at the S/C and I/P implantation sites in order to further elucidate the mechanisms of tissue-implant interaction. Eighteen male albino rats were randomly divided into three experimental groups (n = 5/group) and one control (n = 3). Animals in Groups I, II, and III were implanted with the ALCAP, HA, and TCP ceramics, respectively. Animals in Group IV were not implanted and acted as the control group. At 90 days post-implantation, the animals in all groups were euthanized and the fibrous tissue surrounding the ceramic devices and internal organs were harvested. After routine histological processing, sections of tissue was stained with hematoxylin and eosin and evaluated using light microscopy. Analysis of the data revealed the following: 1) The presence of macrophages, neutrophils, fibroblasts, vascularity, and thickness of the fibrous tissue matrix was found to be statistically different among the subcutaneously implanted ceramic groups (ANOVA, p < 0.01). 2) The presence of collagen (S/C) is not statistically different among the three ceramic groups (ANOVA, p = 0.76). 3) The presence of macrophages, neutrophils, fibroblasts, and collagen comprising the fibrous tissue surrounding the ALCAP, HA, and TCP ceramics (I/P), was found to be statistically different (ANOVA, p < 0.01). 4) Vascularity and thickness of the fibrous tissue surrounding the three I/P ceramics were not found to be statistically different among the three ceramic groups (ANOVA, p > 0.05).
本研究特别尝试将该纤维囊的厚度与在皮下（S/C）和肌内（I/P）植入部位围绕ALCAP、HA和TCP陶瓷的纤维囊中所见的各种组织学成分相关联，以进一步阐明组织-植入物相互作用的机制。18只雄性白化大鼠被随机分为三个实验组（每组n = 5）和一个对照组（n = 3）。第一组、第二组和第三组的动物分别植入ALCAP、HA和TCP陶瓷。第四组的动物未植入，作为对照组。植入后90天，对所有组的动物实施安乐死，并采集陶瓷装置周围和内部器官的纤维组织。经过常规组织学处理后，将组织切片用苏木精和伊红染色，并使用光学显微镜进行评估。数据分析结果如下：1）皮下植入陶瓷组之间，巨噬细胞、中性粒细胞、成纤维细胞、血管分布以及纤维组织基质的厚度在统计学上存在差异（方差分析，p < 0.01）。2）三种陶瓷组之间，胶原（S/C）的存在在统计学上无差异（方差分析，p = 0.76）。3）在围绕ALCAP、HA和TCP陶瓷（I/P）的纤维组织中，巨噬细胞、中性粒细胞、成纤维细胞和胶原的存在在统计学上存在差异（方差分析，p < 0.01）。4）三种I/P陶瓷周围纤维组织的血管分布和厚度在三个陶瓷组之间在统计学上无差异（方差分析，p > 0.05）。
