Li J, Yi J, Wang C, Xu P
Department of Neurobiology, Chinese People's Armed Police Force Medical College, Tianjin 300162, China.
Wei Sheng Yan Jiu. 2000 May 30;29(3):135-7.
To study the effect of cadmium on the apoptosis of spermatogenic cells of rat testis and the protective effect of zinc against it. 24 Wistar male rats were divided into 3 groups (cadmium, cadmium and zinc, and control rats). Rats were injected with low-dose CdCl2 (2 mg/kgBW), zinc acetate(ZnAc, 15 mg/kgBW) before and after injected with low-dose CdCl2 and ZnAc(50 mg/kgBW). The control rats were injected with same-dose of 0.9% NaCl. After 7 days, the apoptosis of spermatogenic cells was studied with in situ nick translation(ISNT) technique. Compared with control group, the number of apoptosis spermategenic cells was obviously increased in cadmium group(P < 0.01, t = 3.87), but it was not significantly different in cadmium and zinc rats. Cadmium treatment could accelerate testis apoptosis. Zinc could proven it.
研究镉对大鼠睾丸生精细胞凋亡的影响以及锌对其的保护作用。将24只Wistar雄性大鼠分为3组（镉组、镉锌组和对照组）。给大鼠注射低剂量氯化镉（2mg/kg体重），在注射低剂量氯化镉前后分别注射醋酸锌（ZnAc，15mg/kg体重）和ZnAc（50mg/kg体重）。对照组大鼠注射同剂量的0.9%氯化钠。7天后，采用原位缺口平移（ISNT）技术研究生精细胞的凋亡情况。与对照组相比，镉组生精细胞凋亡数量明显增加（P<0.01，t=3.87），但镉锌组差异不显著。镉处理可加速睾丸凋亡。锌可以预防。
