Zhao Hong-guang, Liu Guang-wei, Liu Shu-chun, Wang Zhi-cheng, Liu Yang, Wang Zhen-qi, Li Cai, Cai Lu, Gong Shou-liang
MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun, Jilin 130021, China.
Zhonghua Nan Ke Xue. 2005 Oct;11(10):735-9.
To explore the changes of cycle and apoptosis of spermatogenic cells and antioxidant capacity of the serum and testis in male rats with diabetes mellitus.
Thirty male rats were divided into two groups, 10 for normal control and 20 for the diabetes group. The rats were injected intraperitoneally with streptozocin (TZ) to develop diabetes, and 12 weeks later, their survival rate and testis weight were recorded. The percentage of G0/G, S and G2/M phases and apoptosis in spermatogenic cells were measured with flow cytometry (FCM). Malondialdehyde (MDA) and nitric oxide (NO) levels, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and NO synthase (NOS) activities in the serum and testis were measured with thiobarbituric acid reactive substances (TBARs), nitric acid reoxidized enzyme, xanthine oxidative enzyme, 5,5 Dithiobis (2,2 nitrobenzoate) (TNB) and visible light photometer methods, respectively.
Twelve weeks after the male rats got diabetes, their survival rate, body weight and testis weight were significantly lower (p < 0.05), and the percentages of G0/G1 phases and apoptotic spermatogenic cells were obviously higher (P < 0.05) than the normal control. At the same time, the percentage of S and G2/M phases spermatogenic cells decreased. So the spermatogenic cells were arrested in G0/G1 phase. In the diabetic rat serum and testis, especially in the testis, MDA levels were distinctly higher and SOD activities were significantly lower than those in the control. Serum GSH-Px activities of the diabetic rats were significantly lower (p < 0.05), while testis GSH-Px activities were significantly higher than those in control group (P < 0.01). NO contents in the serum and testis of the diabetic rats (P < 0.01) increased significantly, particularly the former, while NOS activities in the serum decreased significantly as compared with the control (P < 0.5).
The increase in testis and serum MDA levels and NO contents and the decrease in the antioxidant enzyme activity of the diabetic rats may be relevant to spermatogenic disorder caused by the increase of G0/G1 phases arrest and spermatogenic cells apoptosis.
探讨糖尿病雄性大鼠生精细胞周期和凋亡变化以及血清和睾丸的抗氧化能力。
将30只雄性大鼠分为两组，正常对照组10只，糖尿病组20只。腹腔注射链脲佐菌素（STZ）诱导大鼠患糖尿病，12周后记录其存活率和睾丸重量。采用流式细胞术（FCM）检测生精细胞G0/G1、S和G2/M期百分比及凋亡情况。分别用硫代巴比妥酸反应物（TBARs）、硝酸还原酶、黄嘌呤氧化酶、5,5-二硫代双（2-硝基苯甲酸）（DTNB）和可见光光度计法检测血清和睾丸中丙二醛（MDA）、一氧化氮（NO）水平，超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和一氧化氮合酶（NOS）活性。
雄性大鼠患糖尿病12周后，其存活率、体重和睾丸重量显著降低（p<0.05），G0/G1期百分比和生精细胞凋亡率明显高于正常对照组（P<0.05）。同时，S期和G2/M期生精细胞百分比降低，生精细胞停滞于G0/G1期。糖尿病大鼠血清和睾丸中，尤其是睾丸，MDA水平明显高于对照组，SOD活性显著低于对照组。糖尿病大鼠血清GSH-Px活性显著降低（p<0.05），而睾丸GSH-Px活性显著高于对照组（P<0.01）。糖尿病大鼠血清和睾丸中NO含量显著增加（P<0.01），尤其是血清，而血清中NOS活性与对照组相比显著降低（P<0.5）。
糖尿病大鼠睾丸和血清中MDA水平及NO含量升高以及抗氧化酶活性降低可能与G0/G1期阻滞增加和生精细胞凋亡导致的生精障碍有关。
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