Bousso Philippe, Robey Ellen
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Nat Immunol. 2003 Jun;4(6):579-85. doi: 10.1038/ni928. Epub 2003 May 5.
The cellular dynamics underlying activation of CD8+ T cells by dendritic cells (DCs) in the lymph node are not known. Here we have tracked the behavior of T cells and DCs by subjecting intact lymph nodes to real-time two-photon microscopy. We show that DCs scan at least 500 different T cells per hour in the absence of antigen. Antigen-bearing DCs are highly efficient in recruiting peptide-specific T cells and can engage more than ten T cells simultaneously. The duration of these interactions is of the order of hours, not minutes. The overall avidity of the interaction influences the probability that T cells will be stably captured by DCs, providing a possible basis for T cell competition. Taken together, our results identify the cellular behaviors that promote an efficient CD8+ T cell response in the lymph node.
树突状细胞（DCs）在淋巴结中激活CD8⁺T细胞的细胞动力学尚不清楚。在这里，我们通过对完整的淋巴结进行实时双光子显微镜观察，追踪了T细胞和DCs的行为。我们发现，在没有抗原的情况下，DCs每小时至少扫描500个不同的T细胞。携带抗原的DCs在招募肽特异性T细胞方面非常高效，并且可以同时与十多个T细胞相互作用。这些相互作用的持续时间以小时计，而非分钟。相互作用的总体亲和力影响T细胞被DCs稳定捕获的概率，这为T细胞竞争提供了可能的基础。综上所述，我们的结果确定了促进淋巴结中高效CD8⁺T细胞反应的细胞行为。
