Schroeder Declan C, Oke Joanne, Hall Matthew, Malin Gillian, Wilson William H
Marine Biological Association, Citadel Hill, Plymouth PL1 2PB, UK.
Appl Environ Microbiol. 2003 May;69(5):2484-90. doi: 10.1128/AEM.69.5.2484-2490.2003.
Denaturing gradient gel electrophoresis was used as a molecular tool to determine the diversity and to monitor population dynamics of viruses that infect the globally important coccolithophorid Emiliania huxleyi. We exploited variations in the major capsid protein gene from E. huxleyi-specific viruses to monitor their genetic diversity during an E. huxleyi bloom in a mesocosm experiment off western Norway. We reveal that, despite the presence of several virus genotypes at the start of an E. huxleyi bloom, only a few virus genotypes eventually go on to kill the bloom.
变性梯度凝胶电泳被用作一种分子工具,以确定感染全球重要的颗石藻——赫氏颗石藻(Emiliania huxleyi)的病毒的多样性,并监测其种群动态。我们利用赫氏颗石藻特异性病毒主要衣壳蛋白基因的变异,在挪威西部外海的中尺度生态模拟实验中,监测赫氏颗石藻水华期间病毒的遗传多样性。我们发现,尽管在赫氏颗石藻水华开始时存在几种病毒基因型,但最终只有少数病毒基因型能够杀死水华。