Plymouth Marine Laboratory, Plymouth, UK.
FEMS Microbiol Ecol. 2012 Aug;81(2):315-23. doi: 10.1111/j.1574-6941.2012.01349.x. Epub 2012 Mar 29.
We studied the temporal succession of vertical profiles of Emiliania huxleyi and their specific viruses (EhVs) during the progression of a natural phytoplankton bloom in the North Sea in June 1999. Genotypic richness was assessed by exploiting the variations in a gene encoding a protein with calcium-binding motifs (GPA) for E. huxleyi and in the viral major capsid protein gene for EhVs. Using denaturing gradient gel electrophoresis and sequencing analysis, we showed at least three different E. huxleyi and EhV genotypic profiles during the period of study, revealing a complex, and changing assemblage at the molecular level. Our results also indicate that the dynamics of EhV genotypes reflect fluctuations in abundance of potential E. huxleyi host cells. The presence and concentration of specific EhVs in the area prior to the bloom, or EhVs transported into the area by different water masses, are significant factors affecting the structure and intraspecific succession of E. huxleyi during the phytoplankton bloom.
我们研究了 1999 年 6 月北海自然浮游植物水华过程中，菱形海线藻及其特定病毒（EhV）的垂直分布的时间序列。通过利用编码具有钙结合基序的蛋白（GPA）的基因以及 EhV 的病毒主要衣壳蛋白基因的变化，评估了基因型丰富度。使用变性梯度凝胶电泳和测序分析，我们在研究期间显示了至少三种不同的菱形海线藻和 EhV 基因型谱，揭示了分子水平上复杂且不断变化的组合。我们的结果还表明，EhV 基因型的动态反映了潜在菱形海线藻宿主细胞丰度的波动。在水华发生之前，特定 EhV 在该区域的存在和浓度，或者通过不同水团运入该区域的 EhV，是影响浮游植物水华期间菱形海线藻结构和种内演替的重要因素。
