在微量滴定板中使用对氧敏感的荧光团进行群落水平的生理特征分析。
Community-level physiological profiling performed with an oxygen-sensitive fluorophore in a microtiter plate.
作者信息
Garland Jay L, Roberts Michael S, Levine Lanfang H, Mills Aaron L
机构信息
Dynamac Corporation, Mail Code DYN-3, Kennedy Space Center, FL 32899, USA.
出版信息
Appl Environ Microbiol. 2003 May;69(5):2994-8. doi: 10.1128/AEM.69.5.2994-2998.2003.
Abstract
Community-level physiological profiling based upon fluorometric detection of oxygen consumption was performed on hydroponic rhizosphere and salt marsh litter samples by using substrate levels as low as 50 ppm with incubation times between 5 and 24 h. The rate and extent of response were increased in samples acclimated to specific substrates and were reduced by limiting nitrogen availability in the wells.
摘要
通过使用低至50 ppm的底物水平，并在5至24小时的孵育时间下，基于荧光法检测氧气消耗对水培根际和盐沼凋落物样本进行了群落水平的生理特征分析。适应特定底物的样本中反应速率和程度增加，而孔中氮的可利用性受限则会使其降低。
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