Reid B J, MacLeod C J, Lee P H, Morriss A W, Stokes J D, Semple K T
Department of Environmental Science, Institute of Environmental and Natural Sciences, Lancaster University, Lancaster LA1 4YQ, UK.
FEMS Microbiol Lett. 2001 Mar 15;196(2):141-6. doi: 10.1111/j.1574-6968.2001.tb10555.x.
This paper describes the validation and application of a simple flask-based (14)C-respirometer system designed to assess mineralisation of (14)C-labelled substrates under defined conditions. Validation of this respirometer system indicated stoichiometric CO(2) trapping up to a maximum of 400 micromol of CO(2) (in a single trap). Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were used to measure growth-linked biodegradation of [(14)C]naphthalene to (14)CO(2). A (14)C activity balance of 101.7+/-8.9% (n=6), after 74 h incubation time and 10 respirometer-opening events, indicated the suitability of the system for monitoring substrate mineralisation. This respirometric apparatus was then successfully applied to assess: (i) the PAH catabolism of microbes in a field contaminated soil, where naphthalene and phenanthrene were rapidly mineralised and (ii) soil-associated organic contaminant bioavailability, where increased soil-phenanthrene contact time resulted in a reduction in phenanthrene mineralisation in the soil. The described respirometer system differs from existing respirometer systems in that the CO(2) trap can be removed and replaced quickly and easily. The system is efficient, reproducible, adaptable to many situations, easy to construct and simple to use, it therefore affords advantages over existing systems.
本文描述了一种基于烧瓶的简单（14）C呼吸仪系统的验证和应用，该系统旨在评估在特定条件下（14）C标记底物的矿化作用。该呼吸仪系统的验证表明，化学计量的CO₂捕获量最高可达400微摩尔CO₂（在单个捕集器中）。使用多环芳烃（PAH）降解细菌来测量[（14）C]萘向（14）CO₂的与生长相关的生物降解。在74小时的孵育时间和10次呼吸仪开启事件后，（14）C活性平衡为101.7±8.9%（n = 6），表明该系统适用于监测底物矿化。然后，该呼吸测量装置成功应用于评估：（i）现场污染土壤中微生物的PAH分解代谢，其中萘和菲迅速矿化；（ii）与土壤相关的有机污染物生物可利用性，其中土壤与菲接触时间的增加导致土壤中菲矿化的减少。所描述的呼吸仪系统与现有呼吸仪系统的不同之处在于，CO₂捕集器可以快速轻松地拆卸和更换。该系统高效、可重复、适用于多种情况、易于构建且使用简单，因此比现有系统具有优势。
