Kareem S O, Akpan I, Osho M B
Department of Microbiology, University of Agriculture, P.M.B 2240, Abeokuta, Nigeria.
Bioresour Technol. 2003 Mar;87(1):133-5. doi: 10.1016/s0960-8524(02)00208-0.
A simple method based on precipitation with Calotropis procera latex was developed for the purification of crude enzyme from fermentation broth. C. procera latex (10(-2) dilution) clarified and concentrated the crude amylase of Aspergillus oryzae 4-fold with 97% recovery of the initial amylase activity in the filtrate in a single step operation. The latex was stable at pH < or = 4.5 and there was no significant difference (P < or = 0.05) in the purification potential of the latex at 4 and 28 degrees C. This method could prove useful to developing economies of the tropics where ambient temperature is around 28 degrees C.
开发了一种基于牛角瓜乳胶沉淀的简单方法，用于从发酵液中纯化粗酶。牛角瓜乳胶（10⁻²稀释液）在一步操作中使米曲霉的粗淀粉酶澄清并浓缩了4倍，滤液中初始淀粉酶活性的回收率为97%。该乳胶在pH≤4.5时稳定，在4℃和28℃下乳胶的纯化潜力没有显著差异（P≤0.05）。对于环境温度约为28℃的热带发展中经济体而言，该方法可能有用。
