Jucá Thiago Lustosa, Ramos Márcio Viana, Moreno Frederico Bruno Mendes Batista, Viana de Matos Mayara Patrícia, Marinho-Filho José Delano Barreto, Moreira Renato Azevedo, de Oliveira Monteiro-Moreira Ana Cristina
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Humberto Monte, Campus do Pici, 60451-970 Fortaleza, CE, Brazil.
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Avenue Coronel Nunes de Melo, 1127, Rodolfo Teófilo, 60430-270 Fortaleza, CE, Brazil.
ScientificWorldJournal. 2013 Nov 18;2013:615454. doi: 10.1155/2013/615454. eCollection 2013.
Calotropis procera is a medicinal plant whose pharmacological properties are associated with its latex. Here, the Calotropis procera latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti-inflammatory and toxicity activity. The crude latex was partitioned, yielding five fractions (49.4% hexane, 5.2% dichloromethane, 2.0% ethyl acetate, 2.1% n-butanol, and 41.1% aqueous). Phytochemical screening and spectroscopy analysis revealed that dichloromethane is the most chemically diverse fraction. Triterpenes were detected in both the hexane and dichloromethane fractions, while flavonoids were detected in the dichloromethane and ethyl acetate fractions. These fractions were cytotoxic to cancer cell lines (LD50 0.05 to 3.9 μ g/mL) and lethal to brine shrimp (LD50 10.9 to 65.7 μ g/mL). Reduced neutrophil migration in rats was observed in carrageenan-induced peritonitis for the dichloromethane (67%), ethyl acetate (56%), and aqueous (72%) fractions. A positive reaction with tolidine and ninhydrin suggested that cyclopeptides are in the ethyl acetate fraction. It is therefore concluded that Calotropis procera latex dichloromethane and ethyl acetate fractions exhibit both in vitro and in vivo activities as well as anti-inflammatory properties. Cyclopeptide detection is especially interesting because previous attempts to investigate these low-molecular cyclic amino acid sequences in C. procera have failed.
牛角瓜是一种药用植物，其药理特性与其乳胶有关。在此，对牛角瓜乳胶馏分进行了研究，以试图追踪其植物化学特征并测定其抗炎和毒性活性。将粗乳胶进行分离，得到五个馏分（49.4%己烷、5.2%二氯甲烷、2.0%乙酸乙酯、2.1%正丁醇和41.1%水相）。植物化学筛选和光谱分析表明，二氯甲烷是化学多样性最高的馏分。在己烷和二氯甲烷馏分中均检测到三萜类化合物，而在二氯甲烷和乙酸乙酯馏分中检测到黄酮类化合物。这些馏分对癌细胞系具有细胞毒性（半数致死剂量为0.05至3.9μg/mL），对卤虫具有致死性（半数致死剂量为10.9至65.7μg/mL）。在角叉菜胶诱导的大鼠腹膜炎中，观察到二氯甲烷（67%）、乙酸乙酯（56%）和水相（72%）馏分可减少中性粒细胞迁移。与甲苯胺和茚三酮呈阳性反应表明乙酸乙酯馏分中存在环肽。因此得出结论，牛角瓜乳胶的二氯甲烷和乙酸乙酯馏分具有体外和体内活性以及抗炎特性。环肽检测尤其有趣，因为此前对牛角瓜中这些低分子环状氨基酸序列的研究均未成功。
