Taguchi Takahiro, Akimaru Kunihiro, Hirai Hirohisa, Hirai Yuriko, Mwenda Jason M, Yuri Kazunari
Department of Anatomy, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
Chromosome Res. 2003;11(2):147-52. doi: 10.1023/a:1022867915595.
We isolated a DNA probe, designated MMDYZ1, using a chromosome microdissection technique from the Y chromosome of the Rhesus monkey. The probe obtained from eight whole Y chromosomes shows higher specificity for the Y short arm of the Rhesus monkey, which consists totally of constitutive heterochromatin. Two microclones (MMY#3 and MMY#4) were constructed from the Y-specific primary PCR products. Sequence analysis of these two microclones revealed that both were essentially identical to each other and the sizes were 870 and 686 bp, respectively. From alignment analysis using the Genbank database of primates, the alphoid DNA has the highest affinity with the probe. However, the total composition of this probe has extremely high homology with the Y short arm of the Rhesus monkey, as demonstrated by fluorescence in-situ hybridization (FISH). Comparative FISH-mapping disclosed that this DNA-sequence cluster was located at extremely different sites on the Y chromosome in several species of the Old World monkey. Accordingly, this probe seems to be a high-quality tool, now established for the first time, for investigating Y chromosome evolution of the Old World monkey.
我们使用染色体显微切割技术从恒河猴的Y染色体中分离出一个名为MMDYZ1的DNA探针。从八条完整的Y染色体获得的该探针对恒河猴的Y短臂具有更高的特异性，该短臂完全由组成型异染色质组成。从Y特异性初级PCR产物构建了两个微克隆（MMY#3和MMY#4）。对这两个微克隆的序列分析表明，它们彼此基本相同，大小分别为870和686 bp。通过使用灵长类动物的Genbank数据库进行比对分析，发现α卫星DNA与该探针具有最高的亲和力。然而，如荧光原位杂交（FISH）所示，该探针的总组成与恒河猴的Y短臂具有极高的同源性。比较FISH定位显示，该DNA序列簇位于几种旧世界猴的Y染色体上截然不同的位置。因此，该探针似乎是首次建立的用于研究旧世界猴Y染色体进化的高质量工具。
