在确定更多鼻病毒株5'非编码区的核苷酸序列后，通过基于核酸序列的扩增改进鼻病毒的检测。
Improved detection of rhinoviruses by nucleic acid sequence-based amplification after nucleotide sequence determination of the 5' noncoding regions of additional rhinovirus strains.
作者信息
Loens K, Ieven M, Ursi D, De Laat C, Sillekens P, Oudshoorn P, Goossens H
机构信息
Department of Microbiology, University of Antwerp UIA, Antwerp, Belgium.
出版信息
J Clin Microbiol. 2003 May;41(5):1971-6. doi: 10.1128/JCM.41.5.1971-1976.2003.
Abstract
The isothermal nucleic acid sequence-based amplification (NASBA) system was applied for the detection of rhinoviruses using primers targeted at the 5' noncoding region (5' NCR) of the viral genome. The nucleotide sequence of the 5' NCRs of 34 rhinovirus isolates was determined to map the most conserved regions and design more appropriate primers and probes. The assay amplified RNA extracted from 30 rhinovirus reference strains and 88 rhinovirus isolates, it did not amplify RNA from 49 enterovirus isolates and other respiratory viruses. The assay allows one to discriminate between group A and B rhinoviruses. Sensitivities for the detection of group B and group A rhinoviruses was 20 and 200 50% tissue culture infective doses, respectively.
摘要
基于等温核酸序列扩增（NASBA）系统，使用针对病毒基因组5'非编码区（5'NCR）的引物来检测鼻病毒。测定了34株鼻病毒分离株5'NCR的核苷酸序列，以确定最保守区域，并设计更合适的引物和探针。该检测方法扩增了从30株鼻病毒参考株和88株鼻病毒分离株中提取的RNA，未扩增49株肠道病毒分离株和其他呼吸道病毒的RNA。该检测方法能够区分A组和B组鼻病毒。检测B组和A组鼻病毒的灵敏度分别为20和200 50%组织培养感染剂量。
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本文引用的文献
1
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J Clin Microbiol. 2002 Apr;40(4):1339-45. doi: 10.1128/JCM.40.4.1339-1345.2002.
2
Rhinovirus infections in myelosuppressed adult blood and marrow transplant recipients.骨髓抑制的成年血液和骨髓移植受者中的鼻病毒感染
Clin Infect Dis. 1999 Sep;29(3):528-32. doi: 10.1086/598627.
3
Improved detection of rhinoviruses in clinical samples by using a newly developed nested reverse transcription-PCR assay.通过使用新开发的巢式逆转录-聚合酶链反应检测法改进临床样本中鼻病毒的检测。
J Clin Microbiol. 1999 Mar;37(3):524-30. doi: 10.1128/JCM.37.3.524-530.1999.
4
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J Virol Methods. 1998 Apr;71(2):197-209. doi: 10.1016/s0166-0934(98)00006-8.
5
Viruses and bacteria in the etiology of the common cold.普通感冒病因中的病毒与细菌。
J Clin Microbiol. 1998 Feb;36(2):539-42. doi: 10.1128/JCM.36.2.539-542.1998.
6
Acute viral infections of upper respiratory tract in elderly people living in the community: comparative, prospective, population based study of disease burden.社区老年人群上呼吸道急性病毒感染：基于人群的疾病负担比较性前瞻性研究
BMJ. 1997 Oct 25;315(7115):1060-4. doi: 10.1136/bmj.315.7115.1060.
7
Reactivity and amplification efficiency of the NASBA HIV-1 RNA amplification system with regard to different HIV-1 subtypes.核酸序列依赖性扩增（NASBA）HIV-1 RNA扩增系统针对不同HIV-1亚型的反应性和扩增效率。
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8
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J Virol Methods. 1997 Jun;66(1):139-47. doi: 10.1016/s0166-0934(97)00049-9.
9
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J Clin Microbiol. 1997 Jul;35(7):1791-3. doi: 10.1128/jcm.35.7.1791-1793.1997.
10
Use of NASBA RNA amplification for detection of Mycobacterium leprae in skin biopsies from untreated and treated leprosy patients.使用核酸序列依赖性扩增（NASBA）RNA扩增技术检测未经治疗和经治疗的麻风病患者皮肤活检标本中的麻风分枝杆菌。
Int J Lepr Other Mycobact Dis. 1996 Dec;64(4):396-403.
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