Fausa O
Scand J Gastroenterol. 1976;11(3):229-32.
Total serum bile acid concentrations were studied by an enzymatic-fluorimetric method employing a highly purified 3 alpha-hydroxysteroid dehydrogenase. In 28 control subjects mean total serum bile acid concentration was 2.5 mumoles/1 (S.D. 1.4). In 6 healthy subjects a significant postprandial increase in total serum bile acids occurred with maximal values at 90 and 120 minutes after ingestion of a liquid test meal. The maximal postprandial increase for each subject was 1.5 to 3 times the fasting value. In 7 patients with various hepatobiliary diseases the maximal postprandial elevation of serum bile acids was higher than in the normals, and the duration of serum bile acid elevation was significantly prolonged. In the patients with normal fasting concentration of bile acids the postprandial elevation was also significantly greater than in the controls. A 2-hour postprandial sample seems suitable for the study of the bile acid test meal response for clinical use.
采用高度纯化的3α-羟基类固醇脱氢酶，通过酶荧光法研究血清总胆汁酸浓度。28名对照受试者的血清总胆汁酸平均浓度为2.5微摩尔/升（标准差1.4）。6名健康受试者在摄入液体试验餐后，血清总胆汁酸出现明显的餐后升高，在摄入后90和120分钟达到最大值。每个受试者餐后的最大升高值为空腹值的1.5至3倍。7名患有各种肝胆疾病的患者，餐后血清胆汁酸的最大升高值高于正常人，且血清胆汁酸升高的持续时间明显延长。在空腹胆汁酸浓度正常的患者中，餐后升高也明显大于对照组。餐后2小时的样本似乎适合用于临床研究胆汁酸试验餐反应。
