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新型水稻赤霉素2-氧化酶基因的表达受生物活性赤霉素的稳态调控。

Expression of novel rice gibberellin 2-oxidase gene is under homeostatic regulation by biologically active gibberellins.

作者信息

Sakai Miho, Sakamoto Tomoaki, Saito Tamio, Matsuoka Makoto, Tanaka Hiroshi, Kobayashi Masatomo

机构信息

National Institute of Agrobiological Sciences, Tsukuba, Japan.

出版信息

J Plant Res. 2003 Apr;116(2):161-4. doi: 10.1007/s10265-003-0080-z. Epub 2003 Feb 18.

DOI:10.1007/s10265-003-0080-z
PMID:12736788
Abstract

We have cloned two genes for gibberellin (GA) 2-oxidase from rice ( Oryza sativa L.). Expression of OsGA2ox2 was not observed. The other gene, OsGA2ox3, was expressed in every tissue examined and was enhanced by the application of biologically active GA. Recombinant OsGA2ox3 protein catalyzed the metabolism of GA(1) to GA(8) and GA(20) to GA(29)-catabolite. These results indicate that OsGA2ox3 is involved in the homeostatic regulation of the endogenous level of biologically active GA in rice.

摘要

我们从水稻（Oryza sativa L.）中克隆了两个赤霉素（GA）2-氧化酶基因。未观察到OsGA2ox2的表达。另一个基因OsGA2ox3在所检测的每个组织中均有表达，并通过施加生物活性GA而增强。重组OsGA2ox3蛋白催化GA(1)向GA(8)以及GA(20)向GA(29)-分解代谢物的代谢。这些结果表明OsGA2ox3参与水稻中生物活性GA内源水平的稳态调节。

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