Sadraei H, Asghari G, Hekmatti A A
Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Iran.
J Ethnopharmacol. 2003 Jun;86(2-3):187-90. doi: 10.1016/s0378-8741(03)00077-1.
Hydroalcoholic extract of Pycnocycla spinosa was shown to have spasmolytic action in vitro and antidiarroheal effect in vivo. The hydroalcoholic extract of P. spinosa is composed of alkaloid-, flavonoid- and saponin-rich components. These components were separated by fractional separation technique. The pharmacological objective of this study was to look for relaxant effect of these components of hydroalcoholic extract of P. spinosa on rat isolated ileum contractions. The alkaloid-, flavonoid- and saponin-rich fractions of P. spinosa inhibited the response to 80 mM KCl in a concentration-dependent manner. The alkaloid-rich fraction was the most and saponin-rich fraction the least effective relaxant of ileum contractions. The alkaloid-rich fraction also inhibited the response to acetylcholine (ACh) and 5-hydroxytryptamine (5-HT). From this study, it can be concluded that the antispasmodic action of hydroalcoholic extract of P. spinosa could be due to components in the alkaloid-rich fraction and to a lesser extent, to its flavonoid-rich fraction.
刺环螺水醇提取物在体外显示出解痉作用，在体内具有止泻效果。刺环螺水醇提取物由富含生物碱、黄酮类化合物和皂苷的成分组成。这些成分通过分级分离技术进行分离。本研究的药理学目的是寻找刺环螺水醇提取物的这些成分对大鼠离体回肠收缩的松弛作用。刺环螺富含生物碱、黄酮类化合物和皂苷的部分以浓度依赖的方式抑制对80 mM KCl的反应。富含生物碱的部分是回肠收缩最有效的松弛剂，而富含皂苷的部分效果最差。富含生物碱的部分也抑制对乙酰胆碱（ACh）和5-羟色胺（5-HT）的反应。从这项研究可以得出结论，刺环螺水醇提取物的解痉作用可能归因于富含生物碱部分的成分，在较小程度上归因于其富含黄酮类化合物的部分。
