Walker Edwin B, Disis Mary L Nora
Providence Portland Medical Center, Earle A Chiles Research Institute, Robert W Franz Cancer Research Center, Portland, Oregon 97213, USA.
Int Rev Immunol. 2003 May-Aug;22(3-4):283-319. doi: 10.1080/08830180305226.
Clinical evaluation of therapeutic tumor vaccines has resulted in examination and comparison of the types of immune function assays required to monitor tumor antigen-stimulated T cell effector function in immunized patients. Three of the most commonly used assays include ELISPOT, tetramer assay, and cytokine flow cytometry (CFC). Discussed are the method and principles for each assay and an assessment of important methodological, reagent, and data acquisition issues that are relevant for the accurate and effective use of the assays. The sensitivity and utility of the assays and present arguments advocating their integrated use in future immunomonitoring studies are also discussed.
治疗性肿瘤疫苗的临床评估促使人们对监测免疫患者体内肿瘤抗原刺激的T细胞效应功能所需的免疫功能检测类型进行了检查和比较。三种最常用的检测方法包括酶联免疫斑点法（ELISPOT）、四聚体检测法和细胞因子流式细胞术（CFC）。本文讨论了每种检测方法的方法和原理，并评估了与准确有效使用这些检测方法相关的重要方法学、试剂和数据采集问题。还讨论了这些检测方法的灵敏度和实用性，并提出了支持在未来免疫监测研究中综合使用它们的论据。
