Baratto Maria Camilla, Tattini Massimiliano, Galardi Carlotta, Pinelli Patrizia, Romani Annalisa, Visioli Francesco, Basosi Riccardo, Pogni Rebecca
Dipartimento di Chimica, Università degli Studi di Siena, Via Aldo Moro, 1-53100 Siena, Italy.
Free Radic Res. 2003 Apr;37(4):405-12. doi: 10.1080/1071576031000068618.
The antioxidant properties of galloyl quinic derivatives isolated from Pistacia lentiscus L. leaves have been investigated by means of Electron Paramagnetic Resonance spectroscopy (EPR) and UV-Vis spectrophotometry. Antioxidant properties have been also estimated using the biologically relevant LDL test. The scavenger activities of gallic acid, 5-O-galloyl, 3,5-O-digalloyl, 3,4,5-O-trigalloyl quinic acid derivatives, have been estimated against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide (O2) radical, and hydroxyl (OH) radical. On the whole, the scavenger activity raised as the number of galloyl groups on the quinic acid skeleton increased. The half-inhibition concentrations (IC50) of di- and tri-galloyl derivatives did not exceed 30 microM for all the tested free radicals. All the tested metabolites strongly reduced the oxidation of low-density lipoproteins (LDL), following a trend similar to that observed for the scavenger ability against OH radical.
利用电子顺磁共振光谱法（EPR）和紫外可见分光光度法，对从乳香黄连木叶片中分离得到的没食子酰奎宁衍生物的抗氧化性能进行了研究。还使用具有生物学相关性的低密度脂蛋白（LDL）试验评估了抗氧化性能。已针对1,1-二苯基-2-苦基肼（DPPH）自由基、超氧阴离子（O₂）自由基和羟基（OH）自由基，评估了没食子酸、5-O-没食子酰、3,5-O-二没食子酰、3,4,5-O-三没食子酰奎宁酸衍生物的清除剂活性。总体而言，随着奎宁酸骨架上没食子酰基团数量的增加，清除剂活性增强。对于所有测试的自由基，二没食子酰和三没食子酰衍生物的半抑制浓度（IC₅₀）均不超过30微摩尔。所有测试的代谢产物均能显著降低低密度脂蛋白（LDL）的氧化，其趋势与对OH自由基的清除能力相似。
