Kariuki Simon K, ter Kuile Feiko O, Wannemuehler Kathleen, Terlouw Dianne J, Kolczak Margarette S, Hawley William A, Phillips-Howard Penelope A, Orago Alloys S S, Nahlen Bernard L, Lal Altaf A, Shi Ya Ping
Centre for Vector Biology and Control Research, Kenya Medical Research Institute, Kisumu, Kenya.
Am J Trop Med Hyg. 2003 Apr;68(4 Suppl):61-7.
As part of a community-based group-randomized trial on the impact of permethrin-treated bed nets (ITNs) on malaria in pregnancy in a holoendemic area of western Kenya, we assessed their effects on antibody responses to Plasmodium falciparum pre-erythrocytic antigens (recombinant circumsporozoite protein [CSP] and peptides complimentary to the repeat region of the liver stage antigen-1 [LSA-1]) and blood stage antigen (recombinant C-terminal domain of the merozoite surface protein-1 [MSP-1(19) kD]) in paired maternal/cord plasma samples obtained from 296 deliveries (157 from ITN villages and 139 control villages). Levels of total IgG and IgG subclasses 1-3 to LSA-1 and total IgG and IgG3 to MSP-1 were lower, whereas those of total IgG to CSP were significantly higher in women from ITN villages than those from control villages. In cord plasma, levels of total IgG and IgG2 to LSA-1 and IgG3 to MSP-1 were lower in ITN villages than in control villages, but antibody responses to CSP were similar. Our results suggest that the use of ITNs decreases antibody responses to LSA-1 and MSP-1 antigens in pregnant women with associated reductions in levels of the same antibodies in cord blood. In contrast, ITN use was found to be associated with increased antibody responses to CSP in pregnant women, but had no effect on antibody levels to CSP in cord blood.
作为在肯尼亚西部高度流行地区开展的一项基于社区的群组随机试验的一部分，该试验旨在研究经氯菊酯处理的蚊帐（ITNs）对妊娠疟疾的影响，我们评估了其对296例分娩（157例来自使用ITN的村庄，139例来自对照村庄）所采集的配对母血/脐血样本中针对恶性疟原虫前体抗原（重组环子孢子蛋白[CSP]和与肝期抗原-1[LSA-1]重复区域互补的肽段）和血期抗原（裂殖子表面蛋白-1的重组C端结构域[MSP-1(19) kD]）的抗体反应的影响。与对照村庄的妇女相比，使用ITN村庄的妇女中，针对LSA-1的总IgG及IgG亚类1 - 3和针对MSP-1的总IgG及IgG3水平较低，而针对CSP的总IgG水平显著较高。在脐血中，使用ITN的村庄中针对LSA-1的总IgG和IgG2以及针对MSP-1的IgG3水平低于对照村庄，但对CSP的抗体反应相似。我们的结果表明，使用ITNs会降低孕妇对LSA-1和MSP-1抗原的抗体反应，同时脐血中相同抗体水平也相应降低。相比之下，发现使用ITN与孕妇对CSP的抗体反应增加有关，但对脐血中CSP的抗体水平没有影响。
