Arima T, Miyamoto-Sudo C, Hiroata M, Tanigawa T, Tsuboi S
Acta Med Okayama. 1975 Jun;29(3):209-23.
An abnormal protein with similar antigenic properties to Fc fragments of IgG, was found in the serum and urine of an eleventh case of heavy (Hgamma1) chain disease (Yok). This protein was purified with ammonium sulfate precipitation and by column chromatography of DEAE cellulose, CM cellulose and Sephadex G-200. The purity of the protein obtained was 98.5%. It was crystallized easily, forming thin hexagonal plates of various sizes. The chemical compositions and physical properties of the protein including viscosity, partial specific volume, diffusion constant, sedimentation constant, frictional ratio, extinction coefficient and iso-ionic point are reported.
在第11例重链（Hγ1）病（Yok）患者的血清和尿液中，发现了一种与IgG的Fc片段具有相似抗原特性的异常蛋白。该蛋白通过硫酸铵沉淀以及DEAE纤维素柱色谱、CM纤维素柱色谱和葡聚糖凝胶G - 200进行纯化。所得蛋白的纯度为98.5%。它很容易结晶，形成各种尺寸的薄六边形板。报告了该蛋白的化学组成和物理性质，包括粘度、比容、扩散常数、沉降常数、摩擦比、消光系数和等离子点。
