Marsik Claudia, Mayr Florian, Cardona Francesco, Derhaschnig Ulla, Wagner Oswald F, Jilma Bernd
Departments of Clinical Pharmacology, Institute of Medical and Chemical Laboratory Diagnostic, Vienna University, Vienna, Austria.
Br J Haematol. 2003 May;121(4):653-6. doi: 10.1046/j.1365-2141.2003.04350.x.
The modulation of Toll-like receptors (TLR) 1, 2 and 4 was studied during experimental human endotoxaemia. Healthy volunteers received 2 ng/kg of lipopolysaccharide (LPS) endotoxin (n = 10). TLR1, 2 and 4 expression occurred on monocytes and neutrophils, with monocytes expressing higher baseline levels of TLR2. LPS infusion downmodulated TLR4 expression on neutrophils, with maximal downregulation occurring at 24 h (-62% from baseline; P < 0.03 versus baseline). Monocyte TLRs were upregulated in vivo (TLR1 and 2), and in vitro (TLR1, 2 and 4) 8 h after LPS bolus (P < 0.05 versus baseline). Therefore, neutrophils and monocytes differentially express surface TLRs, and endotoxaemia differentially regulates TLR expression.
在实验性人类内毒素血症期间，对Toll样受体（TLR）1、2和4的调节进行了研究。健康志愿者接受2 ng/kg的脂多糖（LPS）内毒素（n = 10）。TLR1、2和 4在单核细胞和中性粒细胞上表达，单核细胞表达更高的TLR2基线水平。LPS输注下调了中性粒细胞上的TLR4表达，最大下调发生在24小时（比基线降低62%；与基线相比，P < 0.03）。LPS推注后8小时，单核细胞TLR在体内（TLR1和2）和体外（TLR1、2和4）均上调（与基线相比，P < 0.05）。因此，中性粒细胞和单核细胞差异表达表面TLR，并且内毒素血症差异调节TLR表达。
