编码催化麦角硫因生物合成第一步的酶的糖基转移酶基因（mshA）。
The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA).
作者信息
Newton Gerald L, Koledin Teresa, Gorovitz Batia, Rawat Mamta, Fahey Robert C, Av-Gay Yossef
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA.
出版信息
J Bacteriol. 2003 Jun;185(11):3476-9. doi: 10.1128/JB.185.11.3476-3479.2003.
Abstract
Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.
摘要
麦角硫因是大多数放线菌中存在的主要硫醇，由假二糖1D-肌醇2-乙酰氨基-2-脱氧-α-D-吡喃葡萄糖苷（GlcNAc-Ins）产生。对耻垢分枝杆菌的一个转座子突变体进行测序，该突变体被证明缺乏GlcNAc-Ins和麦角硫因，以鉴定一个假定的糖基转移酶基因，命名为mshA。结核分枝杆菌中的直系同源基因Rv0486用于补充突变体表型。
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2
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3
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6
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