Klein Tino, Ling Zhidong, Heimberg Harry, Madsen Ole D, Heller R Scott, Serup Palle
JDRF Center for Beta Cell Therapy in Europe, Department of Developmental Biology, Hagedorn Research Institute, Gentofte, Denmark.
J Histochem Cytochem. 2003 Jun;51(6):697-706. doi: 10.1177/002215540305100601.
In this study we examined the expression of nestin in islets, the exocrine part, and the big ducts of the adult human pancreas by immunofluorescent double staining. Two different anti-nestin antisera in combination with various pancreatic and endothelial markers were employed. Nestin-immunoreactive cells were found in islets and in the exocrine portion. All nestin-positive cells co-expressed the vascular endothelial markers PECAM-1 (CD31), endoglin (CD105), and CD34 as well as vimentin. Endocrine, acinar, and duct cells did not stain for nestin. We also demonstrated that in the area of big pancreatic ducts, nestin-positive cells represent small capillaries scattered in the connective tissue surrounding the duct epithelium and do not reside between the duct cells. We detected nestin-expressing endothelial cells located adjacent to the duct epithelium where endocrine differentiation occurs. We have shown that nestin is expressed by vascular endothelial cells in human pancreas, and therefore it is unlikely that nestin specifically marks a subpopulation of cells representing endocrine progenitors in the adult pancreas.
在本研究中，我们通过免疫荧光双重染色检测了巢蛋白在成人胰腺的胰岛、外分泌部和大导管中的表达。使用了两种不同的抗巢蛋白抗血清，并结合各种胰腺和内皮标记物。在胰岛和外分泌部发现了巢蛋白免疫反应性细胞。所有巢蛋白阳性细胞共表达血管内皮标记物PECAM-1（CD31）、内皮糖蛋白（CD105）、CD34以及波形蛋白。内分泌细胞、腺泡细胞和导管细胞未被巢蛋白染色。我们还证明，在大胰管区域，巢蛋白阳性细胞代表散在于导管上皮周围结缔组织中的小毛细血管，并不存在于导管细胞之间。我们检测到在发生内分泌分化的导管上皮附近存在表达巢蛋白的内皮细胞。我们已经表明巢蛋白在人胰腺的血管内皮细胞中表达，因此巢蛋白不太可能特异性标记成人胰腺中代表内分泌祖细胞的细胞亚群。
