Dingle J T, Horsfield P, Fell H B, Barratt M E
Ann Rheum Dis. 1975 Aug;34(4):303-11. doi: 10.1136/ard.34.4.303.
Explants of articular cartilage from young pigs were maintained in organ culture for 10--16 days, and degradation of matrix was induced by retinol or complement-sufficient antiserum. The percentage breakdown of proteoglycan and collagen (as hydroxyproline release) was measured. The response of the cartilage depended on whether or not the explants were cut so as to include some of the invading marrow ('invasion zone'). In media containing retinol, cartilage lost up to three-quarters of its proteoglycan whether the invasion zone was present or not, but very little of its collagen unless this region was included. In the presence of complement-sufficient anti-serum, however, cartilage without the invasion zone was virtually unaffected, but both proteoglycan and hydroxyproline were released when invasion zone was included; here proteoglycan release began almost immediately, but there was a time-lag of 6--8 days before a substantial amount of hydroxyproline appeared in the medium. Histological examination of sample explants from the experiments supported the biochemical findings. The possible significance of the results in relation to rheumatoid arthritis is discussed.
取幼年猪的关节软骨外植体进行器官培养10 - 16天，通过视黄醇或补体充足的抗血清诱导基质降解。测定蛋白聚糖和胶原蛋白（以羟脯氨酸释放量表示）的分解百分比。软骨的反应取决于外植体是否被切割以包含一些侵入的骨髓（“侵入区”）。在含有视黄醇的培养基中，无论是否存在侵入区，软骨都会损失高达四分之三的蛋白聚糖，但除非包含该区域，否则其胶原蛋白损失很少。然而，在补体充足的抗血清存在下，没有侵入区的软骨几乎不受影响，但当包含侵入区时，蛋白聚糖和羟脯氨酸都会释放；在这里，蛋白聚糖的释放几乎立即开始，但在培养基中出现大量羟脯氨酸之前有6 - 8天的时间延迟。对实验中样本外植体的组织学检查支持了生化结果。讨论了这些结果与类风湿性关节炎相关的可能意义。
