Fell H B
Agents Actions. 1976 Feb;6(1-3):86-90. doi: 10.1007/BF01972189.
Pig cartilage devoid of marrow or synovium is virtually unaffected by rabbit complement-sufficient antiserum to pig erythrocytes (AS + C'), probably because normal cartilage matrix excludes immunoglobulins. When synovium or invading marrow is present, AS + C' causes depletion of the proteoglycan, with consequent entry of IgG into the cartilage, followed by the breakdown of collagen; the chondrocytes assume a fibroblastic form and their reaction with the antiserum is greatly reduced. If degradation of the matrix has not advanced too far, new matrix is regenerated when the explant is transferred to control medium. Experiments on the depletion of pure cartilage by exposure to retinol, indicate that articular cartilage produces enzymes capable of degrading protoglycan but not collagen. The work was done in collaboration with colleagues at the Strangeways Laboratory and in the Division of Immunology, Pathology Department, University of Cambridge.
不含骨髓或滑膜的猪软骨实际上不受兔抗猪红细胞补体充足抗血清（AS + C'）的影响，这可能是因为正常软骨基质会排斥免疫球蛋白。当存在滑膜或侵入的骨髓时，AS + C'会导致蛋白聚糖耗竭，随后IgG进入软骨，接着胶原蛋白分解；软骨细胞呈现成纤维细胞形态，并且它们与抗血清的反应会大大降低。如果基质的降解没有进展得太远，当外植体转移到对照培养基中时会再生出新的基质。通过暴露于视黄醇对纯软骨进行耗竭实验表明，关节软骨会产生能够降解蛋白聚糖但不能降解胶原蛋白的酶。这项工作是与斯特兰奇韦实验室以及剑桥大学病理学系免疫学部门的同事合作完成的。
