Morimoto M, Yamamoto N, Hayashi T
Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University, 1-1677 Yoshida, Yamaguchi 753-8515, Japan.
Parasitol Res. 2003 Jun;90(2):153-6. doi: 10.1007/s00436-001-0548-5. Epub 2003 Feb 21.
The interleukin (IL)-13-mediated goblet cell response is the major host effector system involved in the expulsion of Nippostrongylus brasiliensis. Lactic dehydrogenase virus (LDV) induced higher levels of N. brasiliensis egg production compared with controls, but the effect of LDV infection on worm expulsion of, and goblet cell and IL-13 responses to, N. brasiliensis have not been studied. In this study, the effects of LDV infection on these host responses against N. brasiliensis were examined. Mice with chronic LDV infection showed significantly lower worm expulsion rates than non-LDV-infected mice after N. brasiliensis infection, and there were no significant differences in the ratio of female versus male adult worms between control and LDV-infected mice. The number of goblet cells in LDV-infected mice was significantly lower than that in controls. In addition, the levels of IL-13 gene expression in lymph nodes were significantly lower in LDV-infected mice compared with controls. These results suggest that LDV infection reduces the protective immune responses against N. brasiliensis infection by the suppression of IL-13 production.
白细胞介素（IL）-13介导的杯状细胞反应是参与巴西日圆线虫排出的主要宿主效应系统。与对照组相比，乳酸脱氢酶病毒（LDV）诱导产生的巴西日圆线虫卵水平更高，但尚未研究LDV感染对巴西日圆线虫排出以及杯状细胞和IL-13对其反应的影响。在本研究中，检测了LDV感染对宿主针对巴西日圆线虫这些反应的影响。慢性LDV感染的小鼠在感染巴西日圆线虫后，其驱虫率显著低于未感染LDV的小鼠，且对照组和感染LDV的小鼠之间雌雄成虫比例无显著差异。感染LDV的小鼠中杯状细胞数量显著低于对照组。此外，与对照组相比，感染LDV的小鼠淋巴结中IL-13基因表达水平显著降低。这些结果表明，LDV感染通过抑制IL-13的产生降低了针对巴西日圆线虫感染的保护性免疫反应。
