乙酰化前后的碱性多价胰蛋白酶抑制剂与DNA和羧甲基纤维素的相互作用。

Interaction of basic polyvalent trypsin inhibitor before and after acetylation with DNA and carboxymethylcellulose.

Szopa J

Arch Immunol Ther Exp (Warsz). 1976;24(2):291-304.

Abstract

The interaction of soluble complexes of DNA with basic polyvalent trypsin inhibitor of Kunitz's type before and after acetylation was studied. The ORD and CD spectra indicate that the secondary structure of DNA in complexes with protein undergoes marked alteration. 2. The Raman, ORD, CD and IR spectra showed that secondary structure of unmodified and acetylated polyvalent inhibitor in complexes with polyanoin undergoes marked alteration. Its content of alpha-spirals decreases, and content of beta-structure increases.

摘要

研究了DNA可溶性复合物与乙酰化前后的Kunitz型碱性多价胰蛋白酶抑制剂之间的相互作用。ORD和CD光谱表明，与蛋白质形成复合物的DNA二级结构发生了显著改变。2. 拉曼光谱、ORD光谱、CD光谱和红外光谱表明，未修饰和乙酰化的多价抑制剂与聚阴离子形成复合物时，其二级结构发生了显著改变。其α-螺旋含量降低，β-结构含量增加。