VirR/VirS调控级联反应影响产气荚膜梭菌13型菌株中质粒编码的假定毒力基因的转录。

The VirR/VirS regulatory cascade affects transcription of plasmid-encoded putative virulence genes in Clostridium perfringens strain 13.

作者信息

Ohtani Kaori, Kawsar Hameem I, Okumura Kayo, Hayashi Hideo, Shimizu Tohru

机构信息

Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1, Tennohdai, Ibaraki Tsukuba 305-8575, Japan.

出版信息

FEMS Microbiol Lett. 2003 May 16;222(1):137-41. doi: 10.1016/S0378-1097(03)00255-6.

DOI:10.1016/S0378-1097(03)00255-6

PMID:12757957

Abstract

We analyzed the transcriptional regulation of the putative virulence genes encoded on the plasmid pCP13 from Clostridium perfringens strain 13. The transcription of the beta2-toxin (cpb2) and possible collagen adhesin (cna) genes were regulated in both a positive and negative manner, respectively, by the two-component VirR/VirS system. The secondary regulator of the VirR/VirS system, VR-RNA, also affects the expression of both of these genes in the same fashion as the VirR/VirS system. This indicates that the global regulatory cascade of the VirR/VirS system controls the expression of virulence genes located on the plasmid, as well as those found chromosomally in C. perfringens strain 13.

摘要

我们分析了产气荚膜梭菌13型菌株质粒pCP13上编码的假定毒力基因的转录调控。β2毒素（cpb2）和可能的胶原黏附素（cna）基因的转录分别受到双组分VirR/VirS系统的正向和负向调控。VirR/VirS系统的二级调节因子VR-RNA，也以与VirR/VirS系统相同的方式影响这两个基因的表达。这表明VirR/VirS系统的全局调控级联控制着位于质粒上的毒力基因的表达，以及产气荚膜梭菌13型菌株染色体上发现的那些毒力基因的表达。