脱氧核糖核酸-核糖核酸杂交反应理论
The theory of the deoxyribonucleic acid - ribonucleic acid hybridization reaction.
作者信息
Thomou H, Katsanos N A
出版信息
Biochem J. 1976 Feb 1;153(2):241-7. doi: 10.1042/bj1530241.
A general equation is derived describing data of DNA-RNA hybridization in the presence of a competing self-annealing reaction of RNA. The well known double-reciprocal relation and the Scatchard equation are shown to be limiting cases of this general equation. Some new hybridization data at various temperatures are presented and analysed by using the new equation. The results can only be explained if we assume that the behavior of DNA towards single RNA molecules is the same as that towards the annealed form, (RNA12. The variation of the equilibrium constant of the hybridization reaction with temperature is small, indicating a small heat of reaction. The maximum amount of hybridized RNA at equilibrium appears to be independent of temperature.
推导了一个通用方程，用于描述在RNA存在竞争性自退火反应的情况下DNA-RNA杂交的数据。结果表明，著名的双倒数关系和斯卡查德方程是该通用方程的极限情况。给出了不同温度下的一些新杂交数据，并使用新方程进行了分析。只有当我们假设DNA对单个RNA分子的行为与对退火形式（RNA12）的行为相同时，结果才能得到解释。杂交反应平衡常数随温度的变化很小，表明反应热很小。平衡时杂交RNA的最大量似乎与温度无关。
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