一种直接展示人红细胞膜凝集素结合成分的方法。
A method for the direct demonstration of the lectin-binding components of the human erythrocyte membrane.
作者信息
Tanner M J, Anstee D J
出版信息
Biochem J. 1976 Feb 1;153(2):265-70. doi: 10.1042/bj1530265.
Abstract
- A method which allows the characterization of lectin-binding components is described. This method should be useful in defining the nature and heterogeneity of these components in cell membranes. 2. The method, which we have used on erythrocyte "ghosts", involves the fixation of "ghost" components after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and incubation with purified 125I-labelled lectins. 3. Each of the four lectins used shows an individual pattern of reactivity towards "ghosts" components. Band 3, the major membrane-penetrating glycoprotein, is bound by the lectins from Ricinus communis and Phaseolus vulgaris (phytohaemagglutinin) and by concanavalin A. The major erythrocyte sialoglycoprotein is bound by the lectins from R. communis, P. vulgaris and Maclura aurantiaca. 4. Three of the lectins displays binding for other membrane components, some of which are not demonstratable by conventional protein- and carbohydrate-staining techniques.
摘要
- 本文描述了一种可对凝集素结合成分进行表征的方法。该方法对于确定细胞膜中这些成分的性质和异质性应是有用的。2. 我们在红细胞“血影”上使用的这种方法，包括在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳后固定“血影”成分，并与纯化的¹²⁵I标记凝集素一起孵育。3. 所使用的四种凝集素中的每一种对“血影”成分都显示出独特的反应模式。带3，即主要的穿膜糖蛋白，可被蓖麻凝集素、菜豆凝集素（植物血凝素）和伴刀豆球蛋白A结合。主要的红细胞唾液酸糖蛋白可被蓖麻凝集素、菜豆凝集素和桑橙凝集素结合。4. 其中三种凝集素对其他膜成分有结合作用，其中一些成分用传统的蛋白质和碳水化合物染色技术无法显示。
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