Botham K M, Ghazarian J G, Kream B E, DeLuca H F
Biochemistry. 1976 May 18;15(10):2130-5. doi: 10.1021/bi00655a016.
An inhibitor of chick kidney mitochondrial 25-hydroxyvitamin D3-1-hydroxylase has been isolated from rat serum by ammonium sulfate precipitation, gel filtration, ionexchange chromatography, and preparative polyacrylamide disc gel electrophoresis. The purified protein was shown to contain iron and has a mol wt of 52 000. The protein is indistinguishable on gel electrophoresis from a similar inhibitor found in rat kidney tissue. The physiological significance of the inhibitor is not known; however, it seems possible that it is responsible for the failure to demonstrate in vitro 25-hydroxyvitamin D3-l-hydroxylation with rat and other mammalian tissues.
通过硫酸铵沉淀、凝胶过滤、离子交换色谱和制备性聚丙烯酰胺圆盘凝胶电泳，已从大鼠血清中分离出一种鸡肾线粒体25-羟基维生素D3-1-羟化酶抑制剂。纯化后的蛋白质显示含有铁，分子量为52000。在凝胶电泳中，该蛋白质与在大鼠肾组织中发现的类似抑制剂无法区分。该抑制剂的生理意义尚不清楚；然而，它似乎可能是导致在体外无法用大鼠和其他哺乳动物组织证明25-羟基维生素D3-1-羟化作用的原因。
