Evereklioglu Cem, Sari Ibrahim, Alasehirli Belgin, Güldür Emin, Cengiz Beyhan, Balat Zcan, Bagci Cahit
Department of Ophthalmology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Med Sci Monit. 2003 May;9(5):BR168-73.
Caffeine is frequently used during pregnancy and associated with teratogenic effects, such as low birth weight, hearth and digital defects, cleft palate and abortion with fetal loss. This study investigated histopathologically the effects of caffeine on neonatal rat cornea.
MATERIAL/METHODS: Fifty pregnant Wistar-Albino rats (dams) were randomly divided into five groups, one control and four experimental. Between day 9 and 21 of gestation, group 1 dams (control, n=10) were exposed to intraperitoneal (i.p.) SF daily until delivery. Group 2 (n=10), group 3 (n=10) and group 4 (n=10) dams were treated with i.p. caffeine at doses of 25, 50 and 100 mg/kg/d, respectively, for the same period. Group 5 dams were given caffeine in distilled water in a gavage at a dose of 50 mg/kg/d during the same period. After normal delivery, the litters were killed at postnatal day 1 or 30 and the eyes were enucleated for corneal histopathologic investigation.
Control and group 1 eyes had normal corneal epithelium, regular stromal fibers, descement membrane and monolayer inner corneal endothelium. The remaining experimental litters demonstrated changes, such as vacuolated endothelial cells with proliferation, hyperchromasia, polymorphism, endothelial cell agenesis, increased stromal mitotic activity and focal increase in corneal thickness with widely separated corneal lamellae in the injured area. These changes occurred most often in the litters treated with high doses of caffeine.
Excessive gestational caffeine intake has been shown histopathologically to have some teratogenic effects on newborn rat cornea.
咖啡因在孕期经常被使用，且与致畸作用相关，如低出生体重、心脏和手指缺陷、腭裂以及流产伴胎儿丢失。本研究通过组织病理学方法研究了咖啡因对新生大鼠角膜的影响。
材料/方法：50只怀孕的Wistar - 白化大鼠（母鼠）被随机分为五组，一组为对照组，四组为实验组。在妊娠第9天至21天期间，第1组母鼠（对照组，n = 10）每天腹腔注射生理盐水直至分娩。第2组（n = 10）、第3组（n = 10）和第4组母鼠在同一时期分别以25、50和100 mg/kg/d的剂量腹腔注射咖啡因。第5组母鼠在同一时期以50 mg/kg/d的剂量经口灌胃给予蒸馏水中的咖啡因。正常分娩后，在出生后第1天或第30天处死仔鼠，摘除眼球进行角膜组织病理学检查。
对照组和第1组的眼睛角膜上皮正常，基质纤维规则，后弹力膜和单层角膜内皮正常。其余实验组仔鼠表现出一些变化，如内皮细胞空泡化伴增殖、核深染、多形性、内皮细胞发育不全、基质有丝分裂活性增加以及损伤区域角膜厚度局部增加且角膜板层分离。这些变化最常出现在高剂量咖啡因处理的仔鼠中。
组织病理学研究表明，孕期过量摄入咖啡因对新生大鼠角膜有一定致畸作用。
