Dabrowski Andrzej, Bojarski Krzysztof, Maciejewski Ryszard
2nd Department of General Surgery, Medical University, Lublin, Poland.
Med Sci Monit. 2003 May;9(5):BR183-7.
In this study we set out to examine changes in magnesium concentration in serum and tissues of rat and the direction of its shifts in tissue concentration in the course of experimental pancreatitis.
MATERIAL/METHODS: The experiment was performed on 200 male Wistar rats, weight 250-350 g, according to Heinkel and Aho's method. Four groups of rats were randomly selected: Z-healthy (20), K1-first control (60), K2-second control (60), D-experimental (60). In Group D acute pancreatitis was induced by injection under anesthesia; in Group K2, physiological fluid was injected; in Group K1, only the needle was inserted. At the 6th, 12th, 24th and 48th experimental hours blood and some organs were collected from 15 animals in each group.
The magnesium level in serum and in the organs was within the normal range for the K1 rats, not significantly different from controls. The lowest serum and kidney concentrations of magnesium were observed after 6 and 12 hours in groups K2 and D. The lowest magnesium concentration in pancreas was observed after 12 and 24 hours in the same two groups. The highest magnesium concentration in liver was detected in the K2 and D animals after 6 and 12 hours, and in heart after 12 hours. Similar changes in the serum glucose level were observed in groups K2 and D.
Our results demonstrate that in the course of experimental acute pancreatitis there is a decreased magnesium concentration in blood serum, pancreas and kidney.
在本研究中，我们着手研究大鼠血清和组织中镁浓度的变化以及实验性胰腺炎过程中其组织浓度的变化方向。
材料/方法：按照海因克尔和阿霍的方法，对200只体重250 - 350克的雄性Wistar大鼠进行实验。随机选取四组大鼠：Z - 健康组（20只）、K1 - 第一对照组（60只）、K2 - 第二对照组（60只）、D - 实验组（60只）。在D组中，通过麻醉下注射诱导急性胰腺炎；在K2组中，注射生理液；在K1组中，仅插入针头。在实验的第6、12、24和48小时，从每组15只动物中采集血液和一些器官。
K1组大鼠血清和器官中的镁水平在正常范围内，与对照组无显著差异。K2组和D组在6小时和12小时后观察到血清和肾脏中镁浓度最低。在同一两组中，胰腺中镁浓度在12小时和24小时后最低。K2组和D组动物在6小时和12小时后肝脏中镁浓度最高，心脏在12小时后最高。K2组和D组血清葡萄糖水平也有类似变化。
我们的结果表明，在实验性急性胰腺炎过程中，血清、胰腺和肾脏中的镁浓度降低。
