粗糙脉孢菌中一般氨基酸通透酶活性缺陷型突变体的分离与鉴定。

Isolation and characterization of a mutant of Neurospora crassa deficient in general amino acid permease activity.

作者信息

Rao E Y, Rao T K, Debusk A G

出版信息

Biochim Biophys Acta. 1975 Nov 17;413(1):45-51. doi: 10.1016/0005-2736(75)90057-7.

Abstract

A mutant of Neurospora crassa (pm-nbg27) was isolated on the basis of its resistance of p-fluoro-phenylalanine on ammonium-deficient Vogel's medium. This mutant was found to be devoid of both conidial and post-conidial (after 180 min of preincubation) transport activity of all amino acids. Genetic analysis of pm-nbg27 by crossing it to wild-type (74A) resulted in the predicted segregants exhibiting transport characteristics of pm-n, pm-b, pm-g, pm-nb, pm-ng, pm-bg and parental types. The above observations confirm the postulated general amino acid permease system as well as a single genetic locus control of that activity.

摘要

基于其在缺铵的沃格尔培养基上对对氟苯丙氨酸的抗性，分离出了粗糙脉孢菌的一个突变体（pm - nbg27）。该突变体被发现缺乏所有氨基酸的分生孢子和分生孢子后（预培养180分钟后）转运活性。通过将pm - nbg27与野生型（74A）杂交对其进行遗传分析，结果预测分离出的菌株表现出pm - n、pm - b、pm - g、pm - nb、pm - ng、pm - bg和亲本类型的转运特征。上述观察结果证实了假定的通用氨基酸通透酶系统以及该活性的单一基因座控制。