Sanchez S, Martinez L, Mora J
J Bacteriol. 1972 Oct;112(1):276-84. doi: 10.1128/jb.112.1.276-284.1972.
Mutants of Neurospora crassa, selected as resistant to l-canavanine and l-thialysine, are partially deficient in the uptake of basic amino acids. Neutral amino acids completely inhibit uptake of basic amino acids, and this inhibition is dependent on the activity of a neutral amino acid permease. In contradistinction, mutants resistant to 4-methyl-dl-tryptophan are partially deficient in the uptake of neutral amino acids. Basic amino acids completely inhibit neutral amino acid uptake, and this inhibition is dependent on the activity of a basic amino acid permease. It is proposed that these specific transport systems compete with a general amino acid permease for some common element. The general permease is also regulated by ammonia, the amino acid pool, or both. The activity of the general permease can be eliminated phenotypically by a high concentration of glycerol or a high temperature. It is also shown that l-citrulline is transported by the neutral amino acid permease and by the general amino acid permease.
被选作对L-刀豆氨酸和L-硫赖氨酸具有抗性的粗糙脉孢菌突变体，在碱性氨基酸摄取方面存在部分缺陷。中性氨基酸会完全抑制碱性氨基酸的摄取，且这种抑制作用依赖于中性氨基酸通透酶的活性。相反，对4-甲基-DL-色氨酸具有抗性的突变体在中性氨基酸摄取方面存在部分缺陷。碱性氨基酸会完全抑制中性氨基酸的摄取，且这种抑制作用依赖于碱性氨基酸通透酶的活性。有人提出，这些特定的转运系统与一种通用氨基酸通透酶竞争某种共同成分。通用通透酶也受氨、氨基酸库或两者的调节。高浓度甘油或高温可在表型上消除通用通透酶的活性。研究还表明，L-瓜氨酸可通过中性氨基酸通透酶和通用氨基酸通透酶进行转运。
