Kravchenko L S, Oksman A Ia, Glubokovskaia I O, Tereshin I M
Biokhimiia. 1976 Jan;41(1):133-42.
Effects of amphotericine B and nistatine on nuclear membrane-bound DNP (DNPm) and free DNP (DNPf) from dog kidney are studied. Intravenous injection of amphotericine B resulted in the increase of binding of DNP particles with nuclear membrane: the content of DNA in DNPm fraction was 50-fold increased. The injection of nistatine did not affect DNP binding with nuclear membrane. Amphotericine B alone increased the protein-DNA ratio and decreased the RNA/DNA ratio in DNPf fraction. Both amphotericine B and nistatine sharply increased the protein/DNA ratio and practically did not change the RNA/DNA ratio in DNPm fraction. Amphotericine B produced considerable changes in temperature denaturation of DNA in DNPo, while nistatine produced no effect. Both antibiotics considerably changed the composition of acid soluble proteins in DNPm and DNPf, non-histone proteins in DNPf, and also they caused the changed and quantitative redistribution of separate lipid components in DNPm lipids. Polyene antibiotics are suggested to effect on animal cell nuclear structures.
研究了两性霉素B和制霉菌素对犬肾中核膜结合型DNP（DNPm）和游离型DNP（DNPf）的影响。静脉注射两性霉素B导致DNP颗粒与核膜的结合增加：DNPm组分中的DNA含量增加了50倍。注射制霉菌素不影响DNP与核膜的结合。单独使用两性霉素B可增加DNPf组分中的蛋白质-DNA比率并降低RNA/DNA比率。两性霉素B和制霉菌素均大幅增加了DNPm组分中的蛋白质/DNA比率，而实际上未改变RNA/DNA比率。两性霉素B使DNPo中DNA的温度变性产生了显著变化，而制霉菌素则无作用。两种抗生素均显著改变了DNPm和DNPf中酸溶性蛋白质的组成、DNPf中非组蛋白的组成，并且它们还导致了DNPm脂质中各脂质成分的定性和定量重新分布。提示多烯抗生素对动物细胞核结构有影响。
