[Changes in the protein composition of nuclear membranes of canine kidneys induced by amphotericin B in vivo].

Intraveneous injection of amphotericin B significantly affects the protein composition of canine kidney nuclear membranes. Electrophoresis in polyacrylamide gel showed that injection of this antibiotic results in a considerable loss or complete disappearance of the histone fractions H1 and H4 (depending on the dose) and a decrease in the content of fraction H3 in the membrane proteins. The fractionation spectrum of other nuclear membrane proteins also shows deviations from normal. One of possible reasons for that is the increased enzymatic degradation of the proteins. It is also probable that the changes in the protein composition of nuclear membranes are due to the presence of sodium deoxycholate in the antibiotic drug and may be accounted for by the nephrotoxic effect of polyenoic antibiotics.