Zerov Iu P
Biokhimiia. 1976 Jan;41(1):35-9.
A new modification of the procedure of the isolation of polyA-containing RNAs is worked out, which makes possible to isolate this RNA fraction free of considerable contamination with rRNA. The administration of 0.0001 M EDTA-Na2 provides the absence of RNA aggregation and prevents non-specific RNA binding on cellulose columns, which takes place when more high EDTA-Na2 concentrations in elution solutions are applied. Under these conditions synthetic polyA in model experiments practically completely binds with cellulose in a broad range of concentrations. It permits to use the procedure described for the preparative isolation of RNA fractions, containing polyA sequences.
已研发出一种分离含多聚腺苷酸（polyA）RNA 程序的新改进方法，该方法能够分离出几乎不受核糖体 RNA（rRNA）严重污染的这种 RNA 组分。加入 0.0001M 的乙二胺四乙酸二钠（EDTA-Na2）可避免 RNA 聚集，并防止在纤维素柱上出现非特异性 RNA 结合，而当洗脱液中使用更高浓度的 EDTA-Na2 时会发生这种非特异性 RNA 结合。在这些条件下，模型实验中的合成多聚腺苷酸在很宽的浓度范围内实际上能完全与纤维素结合。这使得可以使用所描述的程序来制备性分离含有多聚腺苷酸序列的 RNA 组分。
