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[二乙基亚硝胺诱导的原发性肝癌中富含聚腺苷酸的RNA组分]

[Polyadenylic acid rich RNA fractions in primary hepatomas induced with diethylnitrosamine].

作者信息

Ivanov S D, Voronova L A

出版信息

Vopr Onkol. 1976;22(9):52-4.

PMID:188249
Abstract

The column chromatography with unmodified cellulose revealed a polyadenilic acid rich fraction of total RNA from diethylnitrosamine induced primary hepatomas and normal rat liver. It was shown that the quantity of this fraction was increased to 3.15 per cent. The 2.5% polyacrylamide gel electrophoresis of the polyadenylic acid rich fraction indicated its different spectra in tumors and normal rat liver, the main change being in bipolymer fractions with sedimentation coefficient 28 S and higher.

摘要

用未修饰的纤维素进行柱色谱分析,从二乙基亚硝胺诱导的原发性肝癌和正常大鼠肝脏中分离出富含多聚腺苷酸的总RNA组分。结果表明,该组分的含量增加到了3.15%。对富含多聚腺苷酸的组分进行2.5%聚丙烯酰胺凝胶电泳,结果显示其在肿瘤组织和正常大鼠肝脏中的图谱不同,主要变化发生在沉降系数为28S及以上的双聚体组分中。

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