Mocellin Simone, Rossi Carlo R, Pilati Pierluigi, Nitti Donato, Marincola Francesco M
Surgery Branch, Department of Oncological and Surgical Sciences, University of Padova, via Giustiniani 2, 35128 Padova, Italy.
Trends Mol Med. 2003 May;9(5):189-95. doi: 10.1016/s1471-4914(03)00047-9.
In this era of the Human Genome Project, quantitation of gene expression in tumor or host cells is of paramount importance for investigating the gene patterns responsible for cancer development, progression and response or resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that supports its use as a routine bioinstrumentation for gene level measurement. Several applications have already been implemented in the field of cancer research, and others are being validated, showing that this molecular biology tool can provide both researchers and clinicians with precious information concerning the behavior of tumors. Knowledge of the biochemical principles underlying this biotechnology can be of great value to interpret correctly qrt-PCR data.
在人类基因组计划的这个时代，对肿瘤或宿主细胞中的基因表达进行定量分析，对于研究导致癌症发生、发展以及对治疗的反应或抗性的基因模式至关重要。定量实时聚合酶链反应（qrt-PCR）技术最近已达到了一定的灵敏度、准确性和实际操作简便性水平，这支持了其作为基因水平测量的常规生物仪器的应用。该技术在癌症研究领域已经有了一些应用，其他应用也正在得到验证，这表明这种分子生物学工具能够为研究人员和临床医生提供有关肿瘤行为的宝贵信息。了解这种生物技术背后的生化原理对于正确解读qrt-PCR数据具有重要价值。
