Mocellin Simone, Rossi Carlo R, Marincola Francesco M
Surgery Branch, Department of Oncological and Surgical Sciences, University of Padova, Italy.
Arch Immunol Ther Exp (Warsz). 2003;51(5):301-13.
In the era of the Human Genome Project, quantitation of gene expression by tumor/host cells is of paramount importance to investigate gene patterns responsible for cancer development, progression and response/resistance to treatment. Quantitative real-time PCR (qrt-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that support its use as a routine bioinstrumentation for gene level measurement. Several applications have been already implemented in the field of cancer research, and others are being validated, showing that this molecular biology tool can provide both researchers and clinicians with precious information concerning the behaviour of tumors. The knowledge of the biochemical principles underlying this biotechnology can be of great value to correctly interpret qrt-PCR data.
在人类基因组计划时代，通过肿瘤/宿主细胞对基因表达进行定量分析对于研究导致癌症发生、发展以及对治疗反应/耐药性的基因模式至关重要。定量实时PCR（qrt-PCR）技术近来已达到了一定的灵敏度、准确性和实际操作简便性水平，这使其能够作为一种用于基因水平测量的常规生物仪器。该技术在癌症研究领域已经有了一些应用，其他应用也正在得到验证，这表明这种分子生物学工具能够为研究人员和临床医生提供有关肿瘤行为的宝贵信息。了解这项生物技术背后的生化原理对于正确解读qrt-PCR数据具有重要价值。
