Qi Lin, Yang Yong, Xu Ziou, Wang Hongqiu, Pan Zhen, Zhao Dan, Liu Rui, Zhang Haifang, Xie Xiaofang
Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China.
MOE Key Laboratory of Geriatric Diseases and Immunology, Suzhou Medical College, Soochow University, Suzhou, China.
Front Public Health. 2025 Apr 29;13:1531432. doi: 10.3389/fpubh.2025.1531432. eCollection 2025.
Respiratory tract infections (RTIs) caused by various pathogens, including viruses, bacteria, and fungi, pose significant public health challenges worldwide. Understanding the etiology and epidemiology of RTIs is necessary for clinical management, rational drug use, formulation of preventive measures, and vaccine development.
Quantitative real-time PCR was used to detect and analyze respiratory pathogens in outpatients at a hospital in Suzhou, including FluA, FluB, RSV, ADV, HRV, MP, and SARS-CoV-2.
Among the 27,031 respiratory and throat swab samples, the positive rate of virus detection accounts for 25.6%. MP, SARS-CoV-2, and FluA, in particular, showed high positive rates among children, adolescents, and adults. The highest infection rates of RSV, HRV, and ADV were found in patients under 5 years old. High rates of FluA and FluB were observed in patients aged 5-17 and 18-44 years. However, the highest rate of SARS-CoV-2 infection was primarily observed in older adults. Seasonally, the infection rates of SARS-CoV-2 and FluA were highest in spring, FluB, RSV, and ADV in winter, HRV in autumn, and MP in summer and autumn.
By analyzing the results of respiratory virus nucleic acid detection, we can gain a better understanding of the infection status of common respiratory viruses, providing a basis for clinical diagnosis and treatment.
由包括病毒、细菌和真菌在内的各种病原体引起的呼吸道感染（RTIs）在全球范围内构成了重大的公共卫生挑战。了解呼吸道感染的病因和流行病学对于临床管理、合理用药、制定预防措施以及疫苗开发至关重要。
采用定量实时聚合酶链反应（qRT-PCR）检测和分析苏州某医院门诊患者的呼吸道病原体，包括甲型流感病毒（FluA）、乙型流感病毒（FluB）、呼吸道合胞病毒（RSV）、腺病毒（ADV）、人 rhinovirus（HRV）、肺炎支原体（MP）和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）。
在27,031份呼吸道和咽拭子样本中，病毒检测阳性率为25.6%。特别是MP、SARS-CoV-2和FluA在儿童、青少年和成人中显示出较高的阳性率。RSV、HRV和ADV的最高感染率出现在5岁以下的患者中。FluA和FluB的高感染率在5至17岁和18至44岁的患者中观察到。然而，SARS-CoV-2感染率最高主要出现在老年人中。按季节来看，SARS-CoV-2和FluA的感染率在春季最高，FluB、RSV和ADV在冬季最高，HRV在秋季最高，MP在夏季和秋季最高。
通过分析呼吸道病毒核酸检测结果，我们可以更好地了解常见呼吸道病毒的感染状况，为临床诊断和治疗提供依据。
