White S H, Petersen D C, Simon S, Yafuso M
Biophys J. 1976 May;16(5):481-9. doi: 10.1016/S0006-3495(76)85703-7.
The formation of planar bilayer membranes from lipid monolayers as described by Montal and Mueller (Proc. Natl. Acad. Sci. 1972. 69:3561) is analyzed. Bilayers absolutely free of alkane solvents or other nonpolar hydrocarbons can be formed on polytetrafluoroethylene (PTFE) (e.g. Teflon) septa only if certain boundary conditions are satisfied. Measurements have been made of the contact angles between monolayer-coated water and PTFE in the presence and absence of alkane solvents. The measurement suggest that the boundary conditions for formation of stable bilayers can be satisfied only when a nonpolar solvent is present. We conclude that the bilayer must be surrounded by a torus of alkane solvent, petroleum jelly, or silicone grease depending upon the details of technique used to form the bilayer. The non-polar solvent used in the formation of the bilayer may or may not be present in the bilayer depending upon the water solubility and size of the solvent molecule relative to the size of the alkyl chain of the lipid. Detailed sketches describing the formation of bilayers from monolayers are presented.
对蒙塔尔和米勒（《美国国家科学院院刊》，1972年，69卷：3561页）所描述的由脂质单分子层形成平面双层膜的过程进行了分析。只有满足特定的边界条件，才能在聚四氟乙烯（PTFE）（如特氟龙）隔板上形成完全不含烷烃溶剂或其他非极性碳氢化合物的双层膜。已经测量了在有和没有烷烃溶剂存在的情况下，单层包覆水与PTFE之间的接触角。测量结果表明，只有当存在非极性溶剂时，才能满足形成稳定双层膜的边界条件。我们得出结论，根据形成双层膜所采用技术的具体细节，双层膜必须被烷烃溶剂、凡士林或硅脂的环形区域所包围。形成双层膜时使用的非极性溶剂可能存在于双层膜中，也可能不存在，这取决于溶剂分子的水溶性及其相对于脂质烷基链大小的尺寸。文中给出了描述从单分子层形成双层膜的详细示意图。
