Konstantinov Iu M, Liakhovich V V, Panov A V
Biull Eksp Biol Med. 1976 Feb;81(2):166-8.
Studies with liver mitochondria from rats which starved for 48 hours showed the rate of ADP-stimulated respiration to be 20% lower than in the presence of an uncoupler. This effect was eliminated by preincubation of mitochondria with carnitine. Mitochondria from fed rats were characterized by a considerable decrease of states 3 and 4 respiration. In this case carnitine produced no effect. Preincubation of mitochondria from the liver of fed rats with alpha-ketoglutarate resulted in a substantial increase of the states 3 and 4 respiratory rates. There proved to exist at least two types of regulation of adenine nucleotide transport through the inner mitochondrial membrane depending on the metabolic state of the organism, i.e. by inhibition of adenine-nucleotide translocase by cytoplasmic acyl-CoAs and by control of intramitochondrial adenine nucleotide pool.
对饥饿48小时大鼠的肝脏线粒体进行的研究表明，ADP刺激的呼吸速率比存在解偶联剂时低20%。线粒体与肉碱预孵育可消除这种效应。喂食大鼠的线粒体表现为状态3和状态4呼吸的显著降低。在这种情况下，肉碱没有产生影响。喂食大鼠肝脏的线粒体与α-酮戊二酸预孵育导致状态3和状态4呼吸速率大幅增加。事实证明，根据生物体的代谢状态，至少存在两种调节腺嘌呤核苷酸通过线粒体内膜转运的方式，即通过细胞质酰基辅酶A抑制腺嘌呤核苷酸转位酶以及通过控制线粒体内腺嘌呤核苷酸池。
