de Souza Wanderley, da Cunha-e-Silva Narcisa Leal
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-900, Brasil.
Mem Inst Oswaldo Cruz. 2003 Mar;98(2):151-70. doi: 10.1590/s0074-02762003000200001. Epub 2003 May 15.
Cell fractionation, a methodological strategy for obtaining purified organelle preparations, has been applied successfully to parasitic protozoa by a number of investigators. Here we present and discuss the work of several groups that have obtained highly purified subcellular fractions from trypanosomatids, Apicomplexa and trichomonads, and whose work have added substantially to our knowledge of the cell biology of these parasites.
细胞分级分离是一种获取纯化细胞器制剂的方法策略，许多研究人员已成功将其应用于寄生原生动物。在此，我们展示并讨论了几个研究小组的工作，这些小组从锥虫、顶复门原虫和毛滴虫中获得了高度纯化的亚细胞组分，他们的工作极大地增进了我们对这些寄生虫细胞生物学的了解。
