Schindler Stefanie, Bristow Adrian, Cartmell Tammy, Hartung Thomas, Fennrich Stefan
Biochemical Pharmacology, University of Konstanz.
ALTEX. 2003;20(2):59-63.
A comparison between humans and rabbits was performed based on stimulation of whole blood with well-known pyrogens from Gram-negative and Gram-positive bacteria, such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. The reactivity was measured as release of IL-1 beta and IL-8 by ELISA. The reactivity of the two species towards LPS was found to be similar, whereas their reactivity towards LTA differed considerably. Differences between the levels of IL-1 beta and IL-8 release were observed in both species. This finding suggests that the In vitro Pyrogen Test (IPT) which uses human blood to detect contaminations, e.g. of injectable drugs, might predict the human reaction to the contamination better than the "gold standard" rabbit pyrogen test.
基于分别用革兰氏阴性菌和革兰氏阳性菌的著名热原（如脂多糖（LPS）和脂磷壁酸（LTA））刺激全血，对人和兔子进行了比较。通过酶联免疫吸附测定法（ELISA）将反应性测定为白细胞介素-1β（IL-1β）和白细胞介素-8（IL-8）的释放量。发现这两个物种对LPS的反应性相似，而它们对LTA的反应性则有很大差异。在两个物种中均观察到IL-1β和IL-8释放水平的差异。这一发现表明，使用人血检测污染物（例如注射用药物的污染物）的体外热原试验（IPT）可能比“金标准”兔热原试验能更好地预测人体对污染物的反应。
