Lemancewicz Dorota, Dziecioł Janusz, Kłoczko Janusz, Litwiejko-Pietryńczak Elzbieta, Bogusłowicz Wanda
Department of Human Anatomy, Medical Academy of Białystok, Poland.
Folia Morphol (Warsz). 2003 Feb;62(1):51-6.
The aim of the study was an estimation of the histomorphometry of megakaryocytes (MK) in bone marrow in selected myeloproliferative and lymphoproliferative diseases. Bone marrow specimens were obtained by trephine biopsies from 41 patients with polycythaemia rubra vera (PV), idiopathic myelofibrosis (MF), chronic lymphocytic leukaemia (CLL), hairy cell leukaemia (HCL) and diffuse large B-cell lymphoma (L). Morphometric evaluation was performed using a standard program set MicroImage (OLYMPUS). The greatest number of typical nucleated MK, "naked" nuclei, anucleated cytoplasmic fragments and the largest area were found in PV. The circular deviation factor of MK and their nuclei increased in all cases. The greatest number of clusters was observed in PV and HCL. A significant increase in the number of dysplastic and "naked" nuclei of MK was noted in all selected haematological diseases. The presence of neoplastic cells in bone marrow increased the morphological changes in MK. Quantitative and morphometrical significant differentiation of MK in separate microscopic field in the same slides confirms the necessity of performing trephine biopsies in each patient with haematological disorders.
本研究旨在评估某些骨髓增殖性疾病和淋巴细胞增殖性疾病患者骨髓中巨核细胞（MK）的组织形态计量学特征。通过环钻活检从41例真性红细胞增多症（PV）、原发性骨髓纤维化（MF）、慢性淋巴细胞白血病（CLL）、毛细胞白血病（HCL）和弥漫性大B细胞淋巴瘤（L）患者获取骨髓标本。使用标准程序集MicroImage（OLYMPUS）进行形态计量学评估。在PV中发现典型有核MK、“裸核”、无核细胞质片段的数量最多，面积最大。在所有病例中，MK及其细胞核的圆形偏差因子均增加。在PV和HCL中观察到的簇数量最多。在所有选定的血液系统疾病中，均注意到MK发育异常和“裸核”数量显著增加。骨髓中肿瘤细胞的存在增加了MK的形态学变化。同一载玻片不同显微镜视野中MK的定量和形态计量学显著差异证实，对每位血液系统疾病患者进行环钻活检是必要的。
